Multiplex Fluorescent IHC Staining Kit

Immunohistochemical analysis of paraffin embedded rat brain tissue slide using IHCT003 (Five Color mIHC Flourescence Kit)



Immunohistochemical analysis of paraffin embedded human brain tissue slide using IHCT003 (Five Color mIHC Flourescence Kit)



Immunohistochemical analysis of paraffin embedded mouse brain tissue slide using IHCT003 (Five Color mIHC Flourescence Kit)

 

Cat. No.: IHCT003
Detection method: Fluorescent
Section type: FFPE
Size: 100T
Storage and Stability: Store at -20°C stable for 12 months.

GENERAL INFORMATION

Component	Size	Storage
AbByFluor® 520-tyramide	5 ml (Ready to use)	-20°C
AbByFluor® 570-tyramide	5 ml (Ready to use)	-20°C
AbByFluor® 620-tyramide	5 ml (Ready to use)	-20°C
AbByFluor® 690-tyramide	5 ml (Ready to use)	-20°C
TSA+ enhancer	10 μL x 4	-20°C

Principle
Tyramide signal amplification (TSA) technology is an enzyme-based detection method that employs the catalytic activity of horseradish peroxidase (HRP) to help label a target protein in situ. The underlying principle of this approach is that in the presence of hydrogen peroxide, HRP converts a fluorescence-labeled tyramide substrate into a reactive form that covalently binds to tyrosine residues on proteins at or near the HRP. As a result, TSA enables the concentration of fluorescently labeled tyramide at or near the protein of interest, thereby significantly boosting the fluorescence signal and enhancing detection sensitivity.
The covalent bonds formed through this process enable multiple target proteins to be sequentially stained using TSA. Each staining round involves the application of specific antibodies to a protein of interest followed by thoroughly washing to remove the antibodies. However, the covalently bonded tyramide label remains intact.
This process allows simultaneous detection of all biomarkers of interest upon the completion of the assay.
Importantly, each staining cycle engages only one type of primary antibodies, efficiently eliminating concerns of cross-reaction between different antibodies. Moreover, the removal of antibodies before each staining cycle enables the use of primary antibodies from the same species in the same samples.
The fluorescence labeled tyramide provided in this kit can be used individually or in various combinations, making it suitable for single, double, or multiple labeling.

https://www.biossusa.com/products/ihct003